dPCR Mutation Assay PIK3CA 86044 Human

Product Specification

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0000216

Species

Human (Homo sapiens)

Gene Symbol

PIK3CA

Gene aliases

PI3K

Ensembl Gene ID

ENSG00000121879

Entrez Gene ID

5290

Genomic Mutation ID (COSV by COSMIC)

COSV55881636

Legacy Mutation ID (COSM by COSMIC)

COSM86044

Amino Acid Change

p.E365K

Nucleotide Change

c.1093G>A

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

TATATTTGAGTCTATCGAGTGTGTGCATATGTGTATGTTGAGTGTATACATTAGTATATACCTACTTTTTTCTTTTAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCCAGGTAAGGAAGT

Amplicon length

154

Recommended Restriction Enzyme

AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Product Resources

Validation Data

File Size: 101 KBLanguage: English

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Introducing the dPCR Mutation Assay PIK3CA 86044 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay PIK3CA 86044 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay PIK3CA 86044 Human and elevate your work to new heights.

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dPCR Mutation Assay PIK3CA 86044 Human

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Product Specification

Product Resources

Resources

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