Assay Nameβ
dPCR Mutation Assay EGFR 144206 Human
GeneGlobe Cat No (Assay ID)β
DMH0000262
Speciesβ
Human (Homo sapiens)
Gene Symbolβ
EGFR
Gene aliasesβ
ERBB1;ERRP;ERBB
Ensembl Gene IDβ
ENSG00000146648
Entrez Gene IDβ
1956
Genomic Mutation ID (COSV by COSMIC)β
COSV51780076
Legacy Mutation ID (COSM by COSMIC)β
COSM144206
Amino Acid Changeβ
p.L747_E749del
Nucleotide Changeβ
c.2239_2247del
Wildtype Alleleβ
ATTAAGAGAA
Mutant Alleleβ
A
Mutation Strandβ
+
Mutant descriptionβ
Substitution - Missense
Positive Control Sequenceβ
GTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTT
Amplicon lengthβ
93
Recommended Restriction Enzymeβ
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validatedβ
dPCR wet-lab validated
Probe Fluorophoreβ
FAM/HEX or ATTO550/ROX
Quencherβ
Iowa Black
Primer Purificationβ
Desalted
Probe Purificationβ
HPLC