dPCR Mutation Assay KRAS 522 Human

Product Specification

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0001055

Species

Human (Homo sapiens)

Gene Symbol

KRAS

Gene aliases

KRAS1;K-Ras4B;KRAS2

Ensembl Gene ID

ENSG00000133703

Entrez Gene ID

3845

Genomic Mutation ID (COSV by COSMIC)

COSV55497479

Legacy Mutation ID (COSM by COSMIC)

COSM522

Amino Acid Change

p.G12A

Nucleotide Change

c.35G>C

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

ATCAAAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAA

Amplicon length

165

Recommended Restriction Enzyme

CviQI,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Introducing the dPCR Mutation Assay KRAS 522 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay KRAS 522 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay KRAS 522 Human and elevate your work to new heights.

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dPCR Mutation Assay KRAS 522 Human

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Product Specification

Resources

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