dPCR LNA Mutation Assays

์•” ๋ฐ ์ข…์–‘ ๋ฐœ์ƒ๊ณผ ๊ด€๋ จ๋œ ํŠน์ • DNA ์—ผ๊ธฐ์„œ์—ด ๋Œ์—ฐ๋ณ€์ด ๊ฒ€์ถœ์šฉ

  • ๋“€ํ”Œ๋ ‰์Šค ๋ถ„์„ ํ•ญ๋ชฉ ๋””์ž์ธ์œผ๋กœ ๋Œ์—ฐ๋ณ€์ด ๋ฐ ์•ผ์ƒํ˜•(wild type) ์„œ์—ด์„ ๊ฒ€์ถœํ•ฉ๋‹ˆ๋‹ค
  • FAM + HEX ๋˜๋Š” Atto 550 + ROX ํ˜•๊ด‘ ์—ผ๋ฃŒ ์กฐํ•ฉ ์„ ํƒ
  • LNA ๊ฐ•ํ™” ํ”„๋ผ์ด๋จธ ๋ฐ ํ”„๋กœ๋ธŒ๋กœ ๋ถ„์„ ํ•ญ๋ชฉ ํŠน์ด์„ฑ๊ณผ ๋ฏผ๊ฐ๋„๊ฐ€ ์ฆ๊ฐ€ํ•ฉ๋‹ˆ๋‹ค
  • ์›จํŠธ ๋žฉ(wet-lab) ํ…Œ์ŠคํŠธ๋ฅผ ๊ฑฐ์นœ 200๊ฐœ ์ด์ƒ์˜ ํ‘œ์ ๋“ค์— ๋Œ€ํ•œ dPCR ๋ถ„์„ ํ•ญ๋ชฉ
  • ์ˆœํ™˜ ์ข…์–‘ DNA, ์•ก์ƒ ์ƒ๊ฒ€, FFPE ๋ฐ ๊ธฐํƒ€ ์กฐ์ง ๊ฒ€์ฒด์™€ ํ•จ๊ป˜ ์‚ฌ์šฉํ•˜๊ธฐ์— ์ ํ•ฉ
  • QIAcuity Probe PCR Kit์™€ ํ•จ๊ป˜ ์‚ฌ์šฉ

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Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)