dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity Probe PCR Kits

Product

Product List9 products

Verification
dPCR wet-lab validated (8)
dPCR wet-lab verified (1)
GeneGlobe ID: DMH0000002 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 474 Human
AA Mutation: p.V600R
Nucleotide Mutation: c.1798_1799delinsAG
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000039 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 477 Human
AA Mutation: p.V600D
Nucleotide Mutation: c.1799_1800delinsAT
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000001 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 473 Human
AA Mutation: p.V600K
Nucleotide Mutation: c.1798_1799delinsAA
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000004 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 476 Human
AA Mutation: p.V600E
Nucleotide Mutation: c.1799T>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000218 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 1130 Human
AA Mutation: p.V600M
Nucleotide Mutation: c.1798G>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0001099 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 1583011 Human
AA Mutation: p.V600R
Nucleotide Mutation: c.1798_1799delinsCG
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000068 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 6137 Human
AA Mutation: p.V600G
Nucleotide Mutation: c.1799T>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000003 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 475 Human
AA Mutation: p.V600E
Nucleotide Mutation: c.1799_1800delinsAA
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000047 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay BRAF 460 Human
AA Mutation: p.G469A
Nucleotide Mutation: c.1406G>C
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)