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Pancreatic cancer researchers face significant challenges from the lack of early detection biomarkers, which often leads to late diagnosis and limited treatment options. The disease's genetic complexity further complicates the development of effective therapies, as pancreatic tumors exhibit diverse mutations that influence their behavior and treatment response.
Digital PCR (dPCR) has emerged as a potent tool in this field, offering ultra-sensitive detection capabilities to identify and quantify minute amounts of DNA. By enabling precise quantification of genetic alterations at a single-molecule level, dPCR can uncover rare variants associated with early-stage pancreatic cancer and aid in identifying potential biomarkers for early detection. This technology supports the elucidation of pancreatic tumors' complex genetic landscape and the development of personalized treatment approaches.
Pancreatic cancer is one of the most lethal malignancies and primarily includes two common forms: pancreatic adenocarcinoma, which accounts for about 90% of cases, and pancreatic neuroendocrine tumors. The progression and prognosis of this disease are influenced by mutations in several key genes, such as KRAS, CDKN2A, TP53 and SMAD4. These mutations not only shed light on the pathogenesis of the disease but also present viable targets for potential therapeutic interventions.
Our collection of dPCR LNA Mutation Assays equips researchers with precise, sensitive tools to explore the molecular underpinnings of pancreatic cancer and advance the identification of biomarkers and novel therapies.
Gene | Mutation Type | Mutation (CDS) | Mutation (AA) | COSMIC ID (COSV) | COSMIC ID (COSM) | Codon | dPCR Mutation Assay |
---|---|---|---|---|---|---|---|
BRAF | Substitution - Missense | c.1798G>A | p.V600M | COSV56075762 | COSM1130 | 600 | DMH0000218 |
BRAF | Substitution - Missense | c.1798_1799delinsAA | p.V600K | COSV56057713 | COSM473 | 600 | DMH0000001 |
BRAF | Substitution - Missense | c.1798_1799delinsAG | p.V600R | COSV56058419 | COSM474 | 600 | DMH0000002 |
BRAF | Substitution - Missense | c.1799T>A | p.V600E | COSV56056643 | COSM476 | 600 | DMH0000004 |
BRAF | Substitution - Missense | c.1799T>G | p.V600G | COSV56080151 | COSM6137 | 600 | DMH0000068 |
BRAF | Substitution - Missense | c.1799_1800delinsAA | p.V600E | COSV56059110 | COSM475 | 600 | DMH0000003 |
BRAF | Substitution - Missense | c.1799_1800delinsAT | p.V600D | COSV56059623 | COSM477 | 600 | DMH0000039 |
PIK3CA | Substitution - Missense | c.3139C>T | p.H1047Y | COSV55876499 | COSM774 | 1047 | DMH0000209 |
PIK3CA | Substitution - Missense | c.3140A>G | p.H1047R | COSV55873195 | COSM775 | 1047 | DMH0000036 |
PIK3CA | Substitution - Missense | c.3140A>T | p.H1047L | COSV55873401 | COSM776 | 1047 | DMH0000062 |
*FDA ‘Medical Devices; Laboratory Developed Tests’ final rule, May 6, 2024 and European Union regulation requirements on ‘In-House Assays’ (Regulation (EU) 2017/746 -IVDR- Art. 5(5))
dPCR LNA Mutation Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.
The QIAcuityDx dPCR System is intended for in vitro diagnostic use, using automated multiplex quantification dPCR technology, for the purpose of providing diagnostic information concerning pathological states.
QIAcuity and QIAcuityDx dPCR instruments are sold under license from Bio-Rad Laboratories, Inc. and exclude rights for use with pediatric applications. The QIAcuityDx medical device is currently under development and will be available in 20 countries in H2 2024.