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GeneGlobe ID: DMH0000039 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay BRAF 477 Human

AA Mutation: p.V600D
Nucleotide Mutation: c.1799_1800delinsAT
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay BRAF 477 Human
GeneGlobe Cat No (Assay ID)​
DMH0000039
Species​
Human (Homo sapiens)
Gene Symbol​
BRAF
Gene aliases​
BRAF1;BRAF-1
Ensembl Gene ID​
ENSG00000157764
Entrez Gene ID​
673
Genomic Mutation ID (COSV by COSMIC)​
COSV56059623
Legacy Mutation ID (COSM by COSMIC)​
COSM477
Amino Acid Change​
p.V600D
Nucleotide Change​
c.1799_1800delinsAT
Wildtype Allele​
TCA
Mutant Allele​
TAT
Mutation Strand​
-
Mutant description​
Substitution - Missense
Positive Control Sequence​
GAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCAT
Amplicon length​
109
Recommended Restriction Enzyme​
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

Validation Data
File Size: 80.62 KBLanguage: English

Resources

Scientific Posters (2)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays
PDF (175KB)
Safety Data Sheets (1)
Safety Data Sheets
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Certificates of Analysis
Introducing the dPCR Mutation Assay BRAF 477 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay BRAF 477 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay BRAF 477 Human and elevate your work to new heights.
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