GeneGlobe ID: DMH0000002 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay BRAF 474 Human

AA Mutation: p.V600R
Nucleotide Mutation: c.1798_1799delinsAG
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay BRAF 474 Human
GeneGlobe Cat No (Assay ID)​
DMH0000002
Species​
Human (Homo sapiens)
Gene Symbol​
BRAF
Gene aliases​
BRAF1;BRAF-1
Ensembl Gene ID​
ENSG00000157764
Entrez Gene ID​
673
Genomic Mutation ID (COSV by COSMIC)​
COSV56058419
Legacy Mutation ID (COSM by COSMIC)​
COSM474
Amino Acid Change​
p.V600R
Nucleotide Change​
c.1798_1799delinsAG
Wildtype Allele​
CAC
Mutant Allele​
CCT
Mutation Strand​
-
Mutant description​
Substitution - Missense
Positive Control Sequence​
GAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCAT
Amplicon length​
109
Recommended Restriction Enzyme​
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Introducing the dPCR Mutation Assay BRAF 474 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay BRAF 474 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay BRAF 474 Human and elevate your work to new heights.