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Help & Additional Considerations

Sample Multiplexing Calculator

This calculator helps you determine the approximate number of samples that can be multiplexed on your sequencing instrument to reach the required coverage for your QIAseq panel. Before starting a sequencing experiment, you should know the coverage (depth) of sequencing you need to achieve. Please refer to “How do I calculate mean coverage?” to determine the coverage you need to achieve for your panel.

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*Based on 2 x 151 bp paired-end read on Illumina platform. How do I calculate mean coverage?
Sample Multiplexing Calculator

Determine the number of samples you can multiplex in a sequencing run for your QIAseq NGS workflow