Our traditional siRNA solutions support a range of loss-of-function experiments, from the knockdown of a single gene of interest, to small numbers of related genes or genes within a common pathway, to genomewide high-throughput screens.
For particularly difficult applications, the Antisense LNA GapmeRs are an excellent alternative to siRNA. For example, many lncRNAs are confined to the nuclear compartment, where they are inefficiently targeted by siRNA. But these lncRNAs remain sensitive to LNA GapmeRs, which function by RNase H-dependent degradation of complementary RNA targets to provide strand-specific knockdown with no RISC-associated, off-target activity. The LNA GapmeRs are active in vivo and in vitro, enabling the analysis RNA function in a wide range of model systems.
LNA-enhanced antisense oligonucleotides for highly effective knockdown of mRNA and lncRNA
For siRNA transfection optimization and positive control experiments in human cells
For siRNA transfection optimization and positive control experiments in mouse or rat cells
For negative control RNAi experiments
For knockdown of a reporter gene