GeneGlobe ID: DMH0000195 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay KRAS 527 Human

AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay KRAS 527 Human
GeneGlobe Cat No (Assay ID)​
DMH0000195
Species​
Human (Homo sapiens)
Gene Symbol​
KRAS
Gene aliases​
KRAS1;K-Ras4B;KRAS2
Ensembl Gene ID​
ENSG00000133703
Entrez Gene ID​
3845
Genomic Mutation ID (COSV by COSMIC)​
COSV55497378
Legacy Mutation ID (COSM by COSMIC)​
COSM527
Amino Acid Change​
p.G13C
Nucleotide Change​
c.37G>T
Wildtype Allele​
C
Mutant Allele​
A
Mutation Strand​
-
Mutant description​
Substitution - Missense
Positive Control Sequence​
CAAAATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAAT
Amplicon length​
90
Recommended Restriction Enzyme​
CviQI,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

Validation Data
File Size: 84.88 KBLanguage: English

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Introducing the dPCR Mutation Assay KRAS 527 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay KRAS 527 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay KRAS 527 Human and elevate your work to new heights.