dPCR Mutation Assay KRAS 518 Human

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Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.