GeneSolution for Entrez gene 5594 consists of:
SI00605990 (FlexiTube siRNA)
SI00605983 (FlexiTube siRNA)
SI00300762 (FlexiTube siRNA)
SI00300755 (FlexiTube siRNA)
Name
FlexiTube GeneSolution GS5594 for MAPK1
Official Name
mitogen-activated protein kinase 1
Species
Human (Homo sapiens)
RefSeq ID
NM_138957, NM_002745
Species
Human (Homo sapiens)
Official Name
mitogen-activated protein kinase 1
Detected Transcripts
NM_138957 (1514 bp), NM_002745 (5916 bp)
Current Target mRNAs
5594|NM_138957 // 5594|NM_002745
Experimental Details
90% Knockdown
Cell Line: HeLa S3
Concentration of transfected siRNA: 100 nM
Transfection reagent:
RNAiFect Transfection ReagentTransfection format: 24 well
Quantification of gene silencing: 48-72 hours post-transfection
RT-PCR type: Two-step
Normalization Against
GAPDH, Cyclophilin
Species
Human (Homo sapiens)
Official Name
mitogen-activated protein kinase 1
Detected Transcripts
NM_002745 (5916 bp)
Current Target mRNAs
5594|NM_002745
Experimental Details
76% Knockdown
Cell Line: HeLa S3
Concentration of transfected siRNA: 25 nM
Transfection reagent:
RNAiFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: Two-step
Normalization Against
GAPDH, Cyclophilin
Species
Human (Homo sapiens)
Official Name
mitogen-activated protein kinase 1
Detected Transcripts
NM_002745 (5916 bp)
Current Target mRNAs
5594|NM_002745
Experimental Details
89% Knockdown
Cell Line: HeLa S3
Concentration of transfected siRNA: 25 nM
Transfection reagent:
RNAiFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin
Species
Human (Homo sapiens)
Official Name
mitogen-activated protein kinase 1
Detected Transcripts
NM_138957 (1514 bp), NM_002745 (5916 bp)
Current Target mRNAs
5594|NM_138957 // 5594|NM_002745
Experimental Details
89% Knockdown
Cell Line: HeLa S3
Concentration of transfected siRNA: 100 nM
Transfection reagent:
RNAiFect Transfection ReagentTransfection format: 24 well
Quantification of gene silencing: 48-72 hours post-transfection
RT-PCR type: Two-step
Normalization Against
GAPDH, Cyclophilin