Functionally verified siRNA directed against Human EPHA3, (NM_005233, NM_182644).
NM_005233

NM_182644

Species
Human (Homo sapiens)
Official Name
EPH receptor A3
Detected Transcripts
NM_005233 (5837 bp), NM_182644 (2684 bp)
Potential Off-Target mRNAs, High Homology
136319|NM_145808|3663|S // 767558|NM_001128619|3663|S
Potential Off - Target mRNAs, 3' UTR Seed Match
2557|NM_000809|AS // 143662|NM_001135091|AS // 143662|NM_001135092|AS // 143662|NM_145650|AS // 347404|NM_001170331|S // 347404|NM_198511|S
Current Target mRNAs
2042|NM_005233 // 2042|NM_182644
Experimental Details
94% Knockdown
Cell Line: HeLa
25
Concentration of transfected siRNA: 5 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin