dPCR Mutation Assay ESR1 4771561 Human | GeneGlobe

Custom Products Analyze Knowledge Services

Unlock Deeper Insights with GeneGlobe Tutorials

Unlock Deeper Insights with GeneGlobe Tutorials

Explore intuitive guides and resources designed to help you get the most out of GeneGlobe. Whether you're designing assays, browsing curated panels, or diving into analysis tools—find practical help to accelerate your research journey.

Check this out!

Product Specification

AA Mutation: p.S463P
Nucleotide Mutation: c.1387T>C
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay ESR1 4771561 Human

GeneGlobe Cat No (Assay ID)

DMH0001101

Species

Human (Homo sapiens)

Gene Symbol

ESR1

Gene aliases

NR3A1;Era;ER-alpha;ESR

Ensembl Gene ID

ENSG00000091831

Entrez Gene ID

2099

Genomic Mutation ID (COSV by COSMIC)

COSV52784970

Legacy Mutation ID (COSM by COSMIC)

COSM4771561

Amino Acid Change

p.S463P

Nucleotide Change

c.1387T>C

Wildtype Allele

T

Mutant Allele

C

Mutation Strand

+

Mutant description

Substitution - Missense

Control Sequence (wt)

GTTACATCCCATGAACACTCTGGGTCTCCTAGACCTCATCCTCTTTGAGCTTCTCTCTCTCACTCTCTCTCTGCGCATTCAGGAGTGTACACATTTCTGTCCAGCACCCTGAAGTCTCTGGAAGAGAAGGACCATATCCACCGAGTCCTGGACAAGATC

Amplicon length

139

Recommended Restriction Enzyme

PvuII,EcoRI,XbaI,HaeIII

Wet-lab verified

dPCR wet-lab verified

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Product Resources

Validation Data

File Size: 70.54 KBLanguage: English

Resources

Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Introducing the dPCR Mutation Assay ESR1 4771561 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Mutation/Variant Detection applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay ESR1 4771561 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Mutation/Variant Detection, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay ESR1 4771561 Human and elevate your work to new heights.

Country and Language

Follow us

Contact us