dPCR Mutation Assay BRAF 475 Human

Product Specification

AA Mutation: p.V600E
Nucleotide Mutation: c.1799_1800delinsAA
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay BRAF 475 Human

GeneGlobe Cat No (Assay ID)

DMH0000003

Species

Human (Homo sapiens)

Gene Symbol

BRAF

Gene aliases

BRAF1;BRAF-1

Ensembl Gene ID

ENSG00000157764

Entrez Gene ID

673

Genomic Mutation ID (COSV by COSMIC)

COSV56059110

Legacy Mutation ID (COSM by COSMIC)

COSM475

Amino Acid Change

p.V600E

Nucleotide Change

c.1799_1800delinsAA

Wildtype Allele

TCA

Mutant Allele

TTT

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

GAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCAT

Amplicon length

109

Recommended Restriction Enzyme

CviQI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Introducing the dPCR Mutation Assay BRAF 475 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay BRAF 475 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay BRAF 475 Human and elevate your work to new heights.

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dPCR Mutation Assay BRAF 475 Human

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Product Specification

Resources

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