Assay Name
dPCR Mutation Assay EGFR 6240 Human
GeneGlobe Cat No (Assay ID)
DMH0000085
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51765492
Legacy Mutation ID (COSM by COSMIC)
COSM6240
Amino Acid Change
p.T790M
Nucleotide Change
c.2369C>T
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
CACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATAC
Amplicon length
154
Recommended Restriction Enzyme
HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC