Assay Name
dPCR Mutation Assay IDH1 28751 Human
GeneGlobe Cat No (Assay ID)
DMH0000228
Species
Human (Homo sapiens)
Gene Symbol
IDH1
Ensembl Gene ID
ENSG00000138413
Entrez Gene ID
3417
Genomic Mutation ID (COSV by COSMIC)
COSV61616571
Legacy Mutation ID (COSM by COSMIC)
COSM28751
Amino Acid Change
p.R132V
Nucleotide Change
c.394_395delinsGT
Wildtype Allele
ACG
Mutant Allele
AAC
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
AATCACATTATTGCCAACATGACTTACTTGATCCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTT
Amplicon length
79
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC