GeneGlobe ID: DMH0000228 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay IDH1 28751 Human

AA Mutation: p.R132V
Nucleotide Mutation: c.394_395delinsGT
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay IDH1 28751 Human
GeneGlobe Cat No (Assay ID)​
DMH0000228
Species​
Human (Homo sapiens)
Gene Symbol​
IDH1
Ensembl Gene ID​
ENSG00000138413
Entrez Gene ID​
3417
Genomic Mutation ID (COSV by COSMIC)​
COSV61616571
Legacy Mutation ID (COSM by COSMIC)​
COSM28751
Amino Acid Change​
p.R132V
Nucleotide Change​
c.394_395delinsGT
Wildtype Allele​
ACG
Mutant Allele​
AAC
Mutation Strand​
-
Mutant description​
Substitution - Missense
Positive Control Sequence​
AATCACATTATTGCCAACATGACTTACTTGATCCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACAAGCCGGGGGATATTTTT
Amplicon length​
79
Recommended Restriction Enzyme​
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Introducing the dPCR Mutation Assay IDH1 28751 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay IDH1 28751 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay IDH1 28751 Human and elevate your work to new heights.