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dPCR LNA Mutation Assay setup.
Detection of BRAF V600E mutation.
GeneGlobe ID: DMH0001098 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay EGFR 4170221 Human

Select a variant to see the price

Product Specification

AA Mutation: p.L747_A750delinsS
Nucleotide Mutation: c.2240_2248del
dPCR wet-lab validated
Assay Name​
dPCR Mutation Assay EGFR 4170221 Human
GeneGlobe Cat No (Assay ID)​
DMH0001098
Species​
Human (Homo sapiens)
Gene Symbol​
EGFR
Gene aliases​
ERBB1;ERRP;ERBB
Ensembl Gene ID​
ENSG00000146648
Entrez Gene ID​
1956
Genomic Mutation ID (COSV by COSMIC)​
COSV51810296
Legacy Mutation ID (COSM by COSMIC)​
COSM4170221
Amino Acid Change​
p.L747_A750delinsS
Nucleotide Change​
c.2240_2248del
Wildtype Allele​
TTAAGAGAAG
Mutant Allele​
T
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
CAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGT
Amplicon length​
88
Recommended Restriction Enzyme​
PvuII,EcoRI,XbaI,CviQI,HaeIII,AluI
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX, ATTO550/ROX or Cy5/ATTO700
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC
Reaction size​
200rxns and 1000rxns

Resources

Scientific Posters (2)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays Quick-Start Protocol
PDF (72KB)
Safety Data Sheets (1)
Safety Data Sheets
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Certificates of Analysis
Introducing the dPCR Mutation Assay EGFR 4170221 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay EGFR 4170221 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay EGFR 4170221 Human and elevate your work to new heights.
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