dPCR Mutation Assay NOTCH1 13053 Human

Custom Products Analyze Knowledge Services

Unlock Deeper Insights with GeneGlobe Tutorials

Explore intuitive guides and resources designed to help you get the most out of GeneGlobe. Whether you're designing assays, browsing curated panels, or diving into analysis tools—find practical help to accelerate your research journey.

Check this out!

Cart

My Account

Product Specification

AA Mutation: p.R1598P
Nucleotide Mutation: c.4793G>C
dPCR wet-lab verified

Assay Name

dPCR Mutation Assay NOTCH1 13053 Human

GeneGlobe Cat No (Assay ID)

DMH0000146

Species

Human (Homo sapiens)

Gene Symbol

NOTCH1

Gene aliases

TAN1

Ensembl Gene ID

ENSG00000148400

Entrez Gene ID

4851

Genomic Mutation ID (COSV by COSMIC)

COSV53027279

Legacy Mutation ID (COSM by COSMIC)

COSM13053

Amino Acid Change

p.R1598P

Nucleotide Change

c.4793G>C

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Control Sequence (wt)

GGCCGTGTGCGTCACGCTTGAAGACCACGTTGGTGTGCAGCACGCGGCTGAGCTCCCGCAGGAAGTGGAAGGAGCTGTTGCGCAGC

Amplicon length

Recommended Restriction Enzyme

CviQI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab verified

dPCR wet-lab verified

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Download Safety Data Sheets for QIAGEN product components.

Introducing the dPCR Mutation Assay NOTCH1 13053 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Mutation/Variant Detection applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay NOTCH1 13053 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Mutation/Variant Detection, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay NOTCH1 13053 Human and elevate your work to new heights.

Country and Language

Explore dPCR & qPCR assay portfolio

Unlock Deeper Insights with GeneGlobe Tutorials

dPCR Mutation Assay NOTCH1 13053 Human

Product Specification

Resources