Oligo Dilution Calculator
This tool can be used to determine how much water or buffer should be added to a stock solution to obtain a desired concentration. Enter values for stock concentration, stock volume and the desired target concentration.
Insights
Formula for Oligo Dilution
This tool uses the following formula to determine the required volume of diluent needed to achieve a desired oligonucleotide concentration:
Required volume (V1) = desired final volume (V2) × desired concentration (M2) / stock concentration (M1)
M1 × V1 = M2 × V2 represents the principle of conservation of mass in the context of dilution, ensuring the moles of solute before and after dilution remain constant.
Applications of the Oligo Dilution Calculator
- Primer Preparation for PCR: The accuracy of primer concentrations directly affects the efficiency and specificity of PCR assays. Our calculator helps in precisely diluting stock primers to optimal concentrations necessary for robust PCR performance.
- Synthetic Gene Assembly: When constructing synthetic genes, multiple oligonucleotides must be assembled at precise molar ratios. The calculator assists in achieving these ratios by accurate dilution, crucial for successful gene synthesis.
- Quantitative PCR Setup: For qPCR, precise primer and probe concentrations are critical for quantification accuracy. The calculator ensures that these components are diluted to the exact concentrations needed for consistent and reproducible results.
- Labeling Reactions: Diluting oligonucleotides to specific concentrations is essential for efficient labeling with fluorescent dyes or radioactive isotopes used in various detection and imaging techniques.