Oligo Resuspension Calculator
This tool can be used to determine how much water or buffer is needed to resuspend a dry lyophilized oligo to obtain your desired final concentration. Enter the values for your stock amount and the desired target concentration. The oligo amount can be found on the datasheet and on the vial of the oligo.
Insights
Formula for Oligo Resuspension
This tool uses the following formula to calculate the necessary volume of diluent (water or buffer) needed to resuspend lyophilized oligonucleotides to a specified concentration:
Required volume (V) = Amount of oligo (A) / Desired concentration (C)
This equation ensures that the resuspended oligonucleotide solution reaches your target concentration by relating the mass of the oligo to the final volume and concentration.
Applications of the Oligo Resuspension Calculator
- Reconstitution of custom synthesized oligos: Custom synthesized oligonucleotides often come in a lyophilized state and must be resuspended properly before use. The calculator assists in determining the correct volume of buffer or water to achieve precise concentrations, vital for the oligo's intended use.
- Preparation of stock solutions for molecular biology experiments: Proper preparation of oligonucleotide stock solutions from lyophilized form is critical for the consistency of molecular assays. This calculator simplifies the conversion of a dry oligo into a usable stock solution at the desired concentration, essential for accurate experimental setups.
- Standardization of reagents for comparative studies: To compare experimental results reliably, it’s crucial to standardize reagents like oligonucleotides across different experiments. This tool ensures that oligos are resuspended to the exact concentrations needed for reproducibility and validity in comparative analyses.