dPCR Mutation Assay WT1 21392 Human

Product Specification

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0000575

Species

Human (Homo sapiens)

Gene Symbol

WT1

Gene aliases

WAGR;WIT-2;AWT1;NPHS4;WT-1;GUD

Ensembl Gene ID

ENSG00000184937

Entrez Gene ID

7490

Genomic Mutation ID (COSV by COSMIC)

COSV60065589

Legacy Mutation ID (COSM by COSMIC)

COSM21392

Amino Acid Change

p.A382Vfs*4

Nucleotide Change

c.1141_1144dup

Wildtype Allele

Mutant Allele

AACCG

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

TTATTGCAGCCTGGGTAAGCACACATGAAGGGGCGTTTCTCACTGGTCTCAGATGCCGACCGTACAAGAGTCGGGGCTACTCCAGGCACACGTCGCACATCCTGCAGGCAGAG

Amplicon length

Recommended Restriction Enzyme

AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Product Resources

Validation Data

File Size: 78.84 KBLanguage: English

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

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dPCR Mutation Assay WT1 21392 Human

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Product Specification

Product Resources

Resources

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