Assay Name
dPCR Mutation Assay EGFR 6210 Human
GeneGlobe Cat No (Assay ID)
DMH0001097
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51768180
Legacy Mutation ID (COSM by COSMIC)
COSM6210
Amino Acid Change
p.L747_T751delinsS
Nucleotide Change
c.2240_2251del
Wildtype Allele
TTAAGAGAAGCAA
Mutant Allele
T
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
CAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGT
Amplicon length
88
Recommended Restriction Enzyme
PvuII,EcoRI,XbaI,CviQI,HaeIII,AluI
Wet-lab validated
in-silico designed
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC