dPCR Mutation Assay EGFR 4170221 Human

Product Specification

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0001098

Species

Human (Homo sapiens)

Gene Symbol

EGFR

Gene aliases

ERBB1;ERRP;ERBB

Ensembl Gene ID

ENSG00000146648

Entrez Gene ID

1956

Genomic Mutation ID (COSV by COSMIC)

COSV51810296

Legacy Mutation ID (COSM by COSMIC)

COSM4170221

Amino Acid Change

p.L747_A750delinsS

Nucleotide Change

c.2240_2248del

Wildtype Allele

TTAAGAGAAG

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

CAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGT

Amplicon length

Recommended Restriction Enzyme

PvuII,EcoRI,XbaI,CviQI,HaeIII,AluI

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

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dPCR Mutation Assay EGFR 4170221 Human

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Product Specification

Resources

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