Assay Name
dPCR Mutation Assay BRAF 1583011 Human
GeneGlobe Cat No (Assay ID)
DMH0001099
Species
Human (Homo sapiens)
Gene Symbol
BRAF
Gene aliases
BRAF1;BRAF-1
Ensembl Gene ID
ENSG00000157764
Entrez Gene ID
673
Genomic Mutation ID (COSV by COSMIC)
COSV56288520
Legacy Mutation ID (COSM by COSMIC)
COSM1583011
Amino Acid Change
p.V600R
Nucleotide Change
c.1798_1799delinsCG
Wildtype Allele
AC
Mutant Allele
CG
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
CCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCATGA
Amplicon length
90
Recommended Restriction Enzyme
PvuII,EcoRI,XbaI,CviQI,HaeIII
Wet-lab validated
in-silico designed
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC