Virulence gene detection assay targeting the bacterial pneumolysin (ply) gene
dPCR wet-lab validated
GeneGlobe Cat.No. (Assay ID)
DMA00664
Target region
Bacterial pneumolysin (ply) gene
Target (NCBI taxonomy ID)
pneumolysin
Template accession
EF413930.1
Application field
Microbial Virulence
Wet-lab tested in singleplex
Yes, tested dye - FAM
Wet-lab tested in multiplex
Yes
Recommended restriction enzyme
EcoRI
Template present in Microbial DNA Positive Control
Yes
Region of Interest
TGGGACAGAAATGGGCAGGATTTAACGGCTCACTTTACCACTAGTATTCCTTTAAAAGGGAATGTTCGTAATCTCTCTGTCAAAATTAGAGAGTGTACCGGGCTTGCCTGGGAATGGTGGCGTACGGTTTATGAAAAAACCGATTTGCCACTAGTGCGTAAGCGGACGATTTCTATTTGGGGAA