Pyrosequencing: PyroMark Q48 Autoprep
For automated Pyrosequencing with advanced methylation, mutation and SNP quantification
Advantages of the PyroMark Q48 over other Pyrosequencing instruments
- Automated protocol requires less manual interaction
- Compact instrument size
- Advanced Reagents for longer read lengths
- Enabled for connectivity
- Touch screen display that guides users through the process
Product List
Hs_RUNX3_04_PM PyroMark CpG assay
GeneGlobe ID: PM00000133 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000020633). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PTPRU_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000371 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000060656). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_TRIT1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000245 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000043514). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_SDF4_02_PM PyroMark CpG assay
GeneGlobe ID: PM00000602 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000186827, ENSG00000078808). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PAX7_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000056 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000009709). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_AC093578.3_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000399 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000065243, ENSG00000237505). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ZC3H11A_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000336 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000058673). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_TNFRSF1B_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000217 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000028137). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_INSRR_02_PM PyroMark CpG assay
GeneGlobe ID: PM00000175 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000198400, ENSG00000027644). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_TTF2_02_PM PyroMark CpG assay
GeneGlobe ID: PM00001288 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116830, ENSG00000236137). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_MIIP_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001197 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116691). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_MFN2_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001190 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116688). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_MRPS15_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001344 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116898). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PRRX1_02_PM PyroMark CpG assay
GeneGlobe ID: PM00000896 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116132). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_SH3GLB1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00000791 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000097033). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ANKRD34A_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001981 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000181039, ENSG00000121851). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_AL445248.3_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001960 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000121775, ENSG00000203325). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ECHDC2_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001918 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000121310, ENSG00000230953, ENSG00000231866). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_DDX59_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001841 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000118197, ENSG00000232257). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_KLHL12_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001533 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000117153). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_BMP8B_01_PM PyroMark CpG assay
GeneGlobe ID: PM00001442 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000116985). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_CEP350_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002702 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000135837). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_KCNK1_02_PM PyroMark CpG assay
GeneGlobe ID: PM00002681 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000135750). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_CDC73_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002625 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000134371). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_BCAN_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002450 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000132692). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_HOOK1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002646 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000134709). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_C1orf159_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002366 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000131591). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ZNF436_03_PM PyroMark CpG assay
GeneGlobe ID: PM00002177 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000125945, ENSG00000187251). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_SUSD4_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003465 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143502). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PIP5K1A_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003374 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143398). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ADAMTSL4_02_PM PyroMark CpG assay
GeneGlobe ID: PM00003367 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143382). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_FAM163A_02_PM PyroMark CpG assay
GeneGlobe ID: PM00003269 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143340). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PIGM_02_PM PyroMark CpG assay
GeneGlobe ID: PM00003234 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143315). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_FAM40A_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003108 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143093). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_BARHL2_03_PM PyroMark CpG assay
GeneGlobe ID: PM00003052 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143032). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_BARHL2_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003038 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143032). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_DMRTA2_05_PM PyroMark CpG assay
GeneGlobe ID: PM00002947 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000142700). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_AL031283.2_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002884 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000142634, ENSG00000228140). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_C1orf224_01_PM PyroMark CpG assay
GeneGlobe ID: PM00002877 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000237276, ENSG00000142632). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ALDH4A1_02_PM PyroMark CpG assay
GeneGlobe ID: PM00004151 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000159423). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_NMNAT2_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003962 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000157064). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ENAH_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003885 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000154380). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ITPKB_04_PM PyroMark CpG assay
GeneGlobe ID: PM00003752 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143772). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_EFNA3_03_PM PyroMark CpG assay
GeneGlobe ID: PM00003556 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143590). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_HAX1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003535 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143575). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PSMD4_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004116 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000159352). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PEX10_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004004 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000157911). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_FBXO28_01_PM PyroMark CpG assay
GeneGlobe ID: PM00003703 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000143756). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_PMVK_03_PM PyroMark CpG assay
GeneGlobe ID: PM00004872 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000163344). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ACP6_03_PM PyroMark CpG assay
GeneGlobe ID: PM00004795 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000162836). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_C1orf58_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004767 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000162819, ENSG00000186063). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_ZNF281_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004620 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000162702). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_C1orf86_02_PM PyroMark CpG assay
GeneGlobe ID: PM00004550 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000162585, ENSG00000215059). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_AL354712.1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004494 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000162494, ENSG00000229350). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_UBE2Q1_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004291 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000160714). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_CKS1B_01_PM PyroMark CpG assay
GeneGlobe ID: PM00004277 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000160691, ENSG00000173207). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_GBA_02_PM PyroMark CpG assay
GeneGlobe ID: PM00005551 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000177628). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_JUN_05_PM PyroMark CpG assay
GeneGlobe ID: PM00005516 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000177606, ENSG00000234807). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_KCNA3_03_PM PyroMark CpG assay
GeneGlobe ID: PM00005474 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000177272). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
Hs_HES3_03_PM PyroMark CpG assay
GeneGlobe ID: PM00005327 | Cat. No.: 978746 | PyroMark Q48 Autoprep: Instrument, reagents and accessories
(ENSG00000173673). PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)
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Brochures and Guides (3)
Automated Pyrosequencing with integrated template preparation for advanced methylation, mutation and SNP quantification
Operating Software (3)
This is the latest version of the PyroMark Q48 Autoprep instrument software. The software may only be downloaded by registered users with a registered PyroMark Q48 Autoprep instrument. Follow the instructions provided in the user manual chapter 5.4 Upgrading the instrument software when updating the software. The user manual can be downloaded from the PyroMark Q48 Autoprep webpage.
PyroMark Q48 Autoprep Software version 2.4.2 is compatible with Windows 7, Windows 10, and Windows 11 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Q48 Autoprep Software license and registered PyroMark Q48 Autoprep instrument. If you do not have a valid software license, contact your QIAGEN Technical Services.
PyroMark Assay Design Software version 2.0.2 is compatible with Windows 7, Windows 10, and Windows 11 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Assay Design Software license. If you do not have a valid software license, contact your QIAGEN sales representative.
Protocols (9)
A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.
Technical Information (5)
User Manuals (3)
Version 1.1
Comprehensive guide to using the PyroMark Q48 Autoprep System for automated pyrosequencing. Covers installation, assay setup, maintenance, and data analysis workflows.
Version 1.0
January 2026
Quick-start guide for designing Pyrosequencing assays with PyroMark Assay Design 2.0 software.
Kit Handbooks (6)
For use with PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems
Shortage of PyroMark Q48 Autoprep Absorber Strips
For quantification of methylation level of the MLH1 gene using PyroMark Q24, PyroMark Q24 Advanced, and PyroMark Q48 Autoprep
For quantification of methylation level of the MGMT gene using PyroMark Q24, PyroMark Q24 Advanced, and PyroMark Q48 Autoprep
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Scientific poster
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Download Safety Data Sheets for QIAGEN product components.
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