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A simple and rapid plate-based workflow

dPCR Microbial DNA Detection Assay for Escherichia coli (stx2)

GeneGlobe ID: DMA00697 | Cat. No.: 250207 | dPCR Microbial DNA Detection Assays

Product Specification

Bacterial detection assay targeting the shiga toxin 2 subunit A (stx2A) gene
dPCR wet-lab verified
Product name​
Escherichia coli (stx2)
GeneGlobe Cat.No. (Assay ID)​
DMA00697
Target type​
Microbial Id
Target region​
Shiga toxin 2 subunit A (stx2A) gene
Target (NCBI taxonomy ID)​
Escherichia coli (562)
Bacillus coli
Bacterium coli commune
Bacterium coli
E. coli
Enterococcus coli
Escherichia/Shigella coli
Template accession​
CP076232.1
Taxonomy​
Bacteria
Application field​
Human Microbiome
Wastewater & Drinking Water Epidemiology
Wet-lab tested in singleplex​
Yes, tested dye - FAM
Wet-lab tested in multiplex​
No
Recommended restriction enzyme​
EcoRI
Template present in Microbial DNA Positive Control​
No
Additional assay information​
Assay based on proven sequences of mericon qPCR system
Region of Interest​
TGAGGTCCACGTCTCCCGGCGTCATCGTATACACAGGAGCAGTTTCAGACAGTGCCTGACGAAATTCTCTCTGTATCTGCCTGAAGCGTAAGGCTTCT
Reaction size​
200rxns

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 207.29 KBLanguage: English

Resources

Available Product Catalog (2)
dPCR Microbial Detection Assay Catalog
PDF (268.58 KB)
Download
dPCR Microbial Detection Assay Catalog
XLSX (92.89 KB)
Download
Brochures and Guides (2)
dPCR Microbial DNA Detection Assays
PDF (405.1KB)
Download
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Making the invisible visible – A versatile workflow for the detection of low-abundance microbes
PDF (2.8MB)
Download
A versatile workflow for the detection of low-abundance microbes
Kit Handbooks (1)
Microbial DNA dPCR Handbook
PDF (449.2KB)
Download
Protocols (3)
Higher-order multiplexing on QIAcuity: 12-plex dPCR capabilities for detailed biological analysis
PDF (563.5KB)
Download
dPCR Microbial DNA Detection Assays and Custom dPCR Microbial Assays Quick-Start Protocol
PDF (675.5KB)
Download
Advancing higher-order multiplex PCR: Overcoming the limitations of qPCR with QIAcuity digital PCR
PDF (3.1MB)
Download
Scientific Posters (1)
Accurate and sensitive detection of microbial DNA and RNA targets using nanoplate dPCR
PDF (322.9KB)
Download
Technical Information (1)
dPCR Microbial DNA Detection Assays - Assay/target list
PDF (200.9KB)
Download
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
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