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Superior sensitivity in real-time RT-PCR.
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Reproducible real-time RT-PCR.

Hs_DEFB127_1_SG QuantiTect Primer Assay

GeneGlobe ID: QT00016912 | Cat. No.: 249900 | QuantiTect Primer Assays
For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tube

Product Specification

(NM_139074). For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tube

NM_139074

NM_139074
GeneGlobe Id​
QT00016912
Official Symbol​
DEFB127 [Human]
Official Name​
defensin, beta 127
Species​
Human (Homo sapiens)
Entrez Gene Id​
140850
Detected transcript(s)​
NM_139074 (536 bp)
Ensembl Transcript ID​
ENST00000202526
Amplified exons*​
1/2
Amplicon Length​
114 (NM_139074)
Dye label / detection​
SYBR Green
Suitable for two-step RT-PCR?​
Yes, bioinformatically validated.
Please use the QuantiFast SYBR Green PCR Kit, QuantiTect SYBR Green PCR Kit, or Rotor-Gene SYBR Green PCR Kit
Suitable for one-step RT-PCR?​
Yes, bioinformatically validated.
Please use the QuantiFast SYBR Green RT-PCR Kit, QuantiTect SYBR Green RT-PCR Kit, or Rotor-Gene SYBR Green RT-PCR Kit

Resources

Brochures and Guides (3)
Flexible RNAi Technologies You Can Rely On - (EN)
PDF (1MB)
Download
RNA Universe brochure
PDF (927.1KB)
Download
Critical Factors for Successful Real-Time PCR - (EN)
PDF (2.2MB)
Download
Kit Handbooks (1)
(EN) - QuantiTect Primer Assay Handbook
PDF (354KB)
Download
For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection
Protocols (1)
QuantiTect Primer Assays with the QIAcuity OneStep Advanced EvaGreen Kit Quick-Start Protocol
PDF (548.7KB)
Download
For the quantification of mRNA targets using the QuantiTect Primer Assays with the QIAcuity OneStep Advanced EvaGreen Kit
Scientific Posters (2)
Quantitative RT-PCR analysis with genomewide, validated primer sets - (EN)
PDF (1.1MB)
Download
Expression profiling of miRNA and mRNA by real-time PCR - (EN)
PDF (435.6KB)
Download
Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
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