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A simple and rapid plate-based workflow

dPCR Microbial DNA Detection Assay for stxB

GeneGlobe ID: DMA00680 | Cat. No.: 250207 | dPCR Microbial DNA Detection Assays

Product Specification

Virulence gene detection assay targeting the bacterial shiga toxin Stx1a subunit B gene
dPCR wet-lab verified
Product name​
stxB
GeneGlobe Cat.No. (Assay ID)​
DMA00680
Target type​
Virulence
Target region​
Bacterial shiga toxin Stx1a subunit B gene
Target (NCBI taxonomy ID)​
Shiga toxin subunit B, receptor binding subunit
Template accession​
EF441595.1
Taxonomy​
Virulence Genes
Application field​
Microbial Virulence
Wet-lab tested in singleplex​
Yes, tested dye - Cy5
Wet-lab tested in multiplex​
Yes
Recommended restriction enzyme​
EcoRI
Template present in Microbial DNA Positive Control​
Yes
Region of Interest​
TGGCGACGCCTGATTGTGTAACTGGAAAGGTGGAGTATACAAAATATAATGATGACGATACCTTTACAGTTAAAGTGGGTGATAAAGAATTATTTACCAACAGATGGAATCTTCAGTCTCTTCTTCTCAGTGCGCAAATTACGGGGATGACTGTAACCATTAAAACTAATGCCTGTCATAATGGAGGGGGATTCAGCGAAG
Reaction size​
200rxns

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 268.25 KBLanguage: English

Resources

Available Product Catalog (2)
dPCR Microbial Detection Assay Catalog
PDF (268.47 KB)
Download
dPCR Microbial Detection Assay Catalog
XLSX (92.9 KB)
Download
Brochures and Guides (2)
dPCR Microbial DNA Detection Assays
PDF (405.1KB)
Download
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Making the invisible visible – A versatile workflow for the detection of low-abundance microbes
PDF (2.8MB)
Download
A versatile workflow for the detection of low-abundance microbes
Kit Handbooks (1)
Microbial DNA dPCR Handbook
PDF (449.2KB)
Download
Protocols (3)
Higher-order multiplexing on QIAcuity: 12-plex dPCR capabilities for detailed biological analysis
PDF (563.5KB)
Download
dPCR Microbial DNA Detection Assays and Custom dPCR Microbial Assays Quick-Start Protocol
PDF (675.5KB)
Download
Advancing higher-order multiplex PCR: Overcoming the limitations of qPCR with QIAcuity digital PCR
PDF (3.1MB)
Download
Scientific Posters (1)
Accurate and sensitive detection of microbial DNA and RNA targets using nanoplate dPCR
PDF (322.9KB)
Download
Technical Information (1)
dPCR Microbial DNA Detection Assays - Assay/target list
PDF (200.9KB)
Download
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
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