Functionally verified siRNA directed against Human DGKG, (NM_001080744, NM_001080745, NM_001346).
NM_001080744

NM_001080745

NM_001346

Species
Human (Homo sapiens)
Official Name
diacylglycerol kinase, gamma 90kDa
Detected Transcripts
NM_001080744 (5743 bp), NM_001080745 (5701 bp), NM_001346 (5818 bp)
Potential Off-Target mRNAs, High Homology
255967|NM_175854|4544|S
Potential Off - Target mRNAs, 3' UTR Seed Match
4685|NM_004540|AS // 10015|NM_001162429|AS // 10015|NM_013374|AS
Current Target mRNAs
1608|NM_001080744 // 1608|NM_001080745 // 1608|NM_001346
Experimental Details
89% Knockdown
Cell Line: HeLa
20
Concentration of transfected siRNA: 5 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin