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A simple and rapid plate-based workflow

dPCR Microbial DNA Detection Assay for stxA

GeneGlobe ID: DMA00679 | Cat. No.: 250207 | dPCR Microbial DNA Detection Assays

Product Specification

Virulence gene detection assay targeting the bacterial shiga toxin Stx1 subunit A gene
dPCR wet-lab validated
Product name​
stxA
GeneGlobe Cat.No. (Assay ID)​
DMA00679
Target type​
Virulence
Target region​
Bacterial shiga toxin Stx1 subunit A gene
Target (NCBI taxonomy ID)​
Shiga toxin subunit A, RNA-N-glycosidase, catalyticsubunit
Template accession​
GQ429156.1
Taxonomy​
Virulence Genes
Application field​
Microbial Virulence
Wet-lab tested in singleplex​
Yes, tested dye - ROX
Wet-lab tested in multiplex​
No
Recommended restriction enzyme​
EcoRI
Template present in Microbial DNA Positive Control​
Yes
Region of Interest​
TTCCAGGTACAACAGCGGTTACATTGTCTGGTGACAGTAGCTATACCACGTTACAGCGTGTTGCAGGGATCAGTCGTACGGGGATGCAGATAAATCGCCATTCGTTGACTACTTCTTATCTGGATTTAATGTCGCATAGTGGAACCTCACTGACGCAGTC
Reaction size​
200rxns

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 262.7 KBLanguage: English

Resources

Available Product Catalog (2)
dPCR Microbial Detection Assay Catalog
PDF (268.14 KB)
dPCR Microbial Detection Assay Catalog
XLSX (92.91 KB)
Quick-Start Protocols (1)
dPCR Microbial DNA Detection Assays and Custom dPCR Microbial Assays Quick-Start Protocol
PDF (675KB)
Application Notes (2)
Advancing higher-order multiplex PCR: Overcoming the limitations of qPCR with QIAcuity digital PCR
PDF (3MB)
Higher-order multiplexing on QIAcuity: 12-plex dPCR capabilities for detailed biological analysis
PDF (563KB)
Technical Information (1)
dPCR Microbial DNA Detection Assays - Assay/target list
PDF (200KB)
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Kit Handbooks (1)
Microbial DNA dPCR Handbook
PDF (449KB)
Scientific Posters (1)
Accurate and sensitive detection of microbial DNA and RNA targets using nanoplate dPCR
PDF (322KB)
Brochures & Guides (2)
dPCR Microbial DNA Detection Assays Product Profile
PDF (405KB)
Detect microbial targets – bacterial, fungal, parasitic, viral, antibiotic resistance and virulence factor genes – using digital PCR
Making the invisible visible
PDF (2MB)
A versatile workflow for the detection of low-abundance microbes
Safety Data Sheets (1)
Safety Data Sheets
Certificates of Analysis (1)
Certificates of Analysis
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