Functionally verified siRNA directed against Human MARK2, (NM_001039468, NM_001039469, NM_001163296, NM_001163297, NM_004954, NM_017490).
Species
Human (Homo sapiens)
Official Name
MAP/microtubule affinity-regulating kinase 2
Detected Transcripts
NM_001039468 (2579 bp), NM_001039469 (4748 bp), NM_001163296 (4541 bp), NM_017490 (4535 bp), NM_001163297 (4511 bp), NM_004954 (4556 bp)
Potential Off-Target mRNAs, High Homology
2214|NM_000569|1949|S // 2214|NM_001127592|1946|S // 2214|NM_001127593|1812|S // 2214|NM_001127595|1794|S // 2214|NM_001127596|1791|S // 2215|NM_000570|1934|S
Current Target mRNAs
2011|NM_001039468 // 2011|NM_001039469 // 2011|NM_001163296 // 2011|NM_017490 // 2011|NM_001163297 // 2011|NM_004954
Experimental Details
84% Knockdown
Cell Line: HeLa
22
Concentration of transfected siRNA: 25 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin