Functionally verified siRNA directed against Human CERK, (NM_022766, NM_182661).
NM_182661

NM_022766

Species
Human (Homo sapiens)
Official Name
ceramide kinase
Detected Transcripts
NM_182661 (3040 bp), NM_022766 (4445 bp)
Potential Off-Target mRNAs, High Homology
57670|NM_001164665|6677|S // 57670|NM_020910|6629|S
Potential Off - Target mRNAs, 3' UTR Seed Match
9669|NM_015904|AS
Current Target mRNAs
64781|NM_182661 // 64781|NM_022766
Experimental Details
91% Knockdown
Cell Line: HeLa
23
Concentration of transfected siRNA: 25 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin