Functionally verified siRNA directed against Human PAFAH1B3, (NM_001145939, NM_001145940, NM_002573).
NM_001145939

NM_001145940

NM_002573

Species
Human (Homo sapiens)
Official Name
platelet activating factor acetylhydrolase 1b catalytic subunit 3
Detected Transcripts
NM_001145939 (1108 bp), NM_001145940 (869 bp), NM_002573 (1086 bp)
Potential Off-Target mRNAs, High Homology
31|NM_198834|3835|S // 31|NM_198836|3402|S // 31|NM_198837|3472|S // 31|NM_198838|3583|S // 31|NM_198839|4509|S
Current Target mRNAs
5050|NM_001145939 // 5050|NM_001145940 // 5050|NM_002573
Experimental Details
97% Knockdown
Cell Line: HeLa S3
0
Concentration of transfected siRNA: 100 nM
Transfection reagent:
RNAiFect Transfection ReagentTransfection format: 24 well
Quantification of gene silencing: 48-72 hours post-transfection
RT-PCR type: Two-step
Normalization Against
GAPDH, Cyclophilin