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Superior sensitivity in real-time RT-PCR.
Time savings in development of real-time RT-PCR assays.
Reproducible real-time RT-PCR.

Hs_PCYT1A_1_SG QuantiTect Primer Assay

GeneGlobe ID: QT00051835 | Cat. No.: 249900 | QuantiTect Primer Assays

Product Specification

(NM_005017). For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tube

NM_005017

NM_005017
GeneGlobe Id​
QT00051835
Official Symbol​
PCYT1A [Human]
Official Name​
phosphate cytidylyltransferase 1, choline, alpha
Species​
Human (Homo sapiens)
Entrez Gene Id​
5130
Detected transcript(s)​
NM_005017 (1677 bp)
Ensembl Transcript ID​
ENST00000292823
Amplified exons*​
1/2/3
Amplicon Length​
99 (NM_005017)
Dye label / detection​
SYBR Green
Suitable for two-step RT-PCR?​
Yes, bioinformatically validated.
Please use the QuantiFast SYBR Green PCR Kit, QuantiTect SYBR Green PCR Kit, or Rotor-Gene SYBR Green PCR Kit
Suitable for one-step RT-PCR?​
Yes, bioinformatically validated.
Please use the QuantiFast SYBR Green RT-PCR Kit, QuantiTect SYBR Green RT-PCR Kit, or Rotor-Gene SYBR Green RT-PCR Kit

Resources

Brochures & Guides (2)
Flexible RNAi Technologies You Can Rely On - (EN)
PDF (1MB)
RNA Universe brochure
PDF (927KB)
Kit Handbooks (1)
(EN) - QuantiTect Primer Assay Handbook
PDF (354KB)
For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection
Quick-Start Protocols (1)
QuantiTect Primer Assays with the QIAcuity OneStep Advanced EvaGreen Kit Quick-Start Protocol
PDF (548KB)
For the quantification of mRNA targets using the QuantiTect Primer Assays with the QIAcuity OneStep Advanced EvaGreen Kit
Articles (1)
Using endogenous control genes in real-time RT-PCR
QuantiTect Primer Assays for housekeeping genes can be used as endogenous controls in qRT-PCR.
Safety Data Sheets (1)
Safety Data Sheets
Scientific Posters (2)
Expression profiling of miRNA and mRNA by real-time PCR - (EN)
PDF (435KB)
Quantitative RT-PCR analysis with genomewide, validated primer sets - (EN)
PDF (1MB)
Gene Expression Analysis (1)
Critical Factors for Successful Real-Time PCR - (EN)
PDF (2MB)
Certificates of Analysis (1)
Certificates of Analysis
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