This calculator helps you determine the approximate number of samples that can be multiplexed on your sequencing instrument to reach the required coverage for your QIAseq panel. Before starting a sequencing experiment, you should know the coverage (depth) of sequencing you need to achieve. Please refer to “How do I calculate mean coverage?” to determine the coverage you need to achieve for your panel.
Determine the number of samples you can multiplex in a sequencing run for your QIAseq NGS workflow