Assay Name
dPCR Mutation Assay IDH1 88208 Human
GeneGlobe Cat No (Assay ID)
DMH0000230
Species
Human (Homo sapiens)
Gene Symbol
IDH1
Ensembl Gene ID
ENSG00000138413
Entrez Gene ID
3417
Genomic Mutation ID (COSV by COSMIC)
COSV61621070
Legacy Mutation ID (COSM by COSMIC)
COSM88208
Amino Acid Change
p.R100Q
Nucleotide Change
c.299G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
ATTTTTGCAGATAATGGCTTCTCTGAAGACCGTGCCACCCAGAATATTTCGTATGGTGCCATTTGGTGATTTCCACATTTGTTTCAACTTGAACTCCTCAACCCTCTTCTCATCAGGAGTGATAGTGGCA
Amplicon length
110
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC