Assay Name
dPCR Mutation Assay EGFR 12378 Human
GeneGlobe Cat No (Assay ID)
DMH0000248
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51769298
Legacy Mutation ID (COSM by COSMIC)
COSM12378
Amino Acid Change
p.D770_N771insG
Nucleotide Change
c.2310_2311insGGT
Wildtype Allele
C
Mutant Allele
CGGT
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TCTCCCTCCCTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGT
Amplicon length
69
Recommended Restriction Enzyme
CviQI,AluI,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC