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dPCR LNA Mutation Assay setup.
Detection of BRAF V600E mutation.
GeneGlobe ID: DMH0000256 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay MET 735 Human

Select a variant to see the price

Product Specification

AA Mutation: p.?
Nucleotide Mutation: c.3083-52_3083-51insCT
dPCR wet-lab validated
Assay Name​
dPCR Mutation Assay MET 735 Human
GeneGlobe Cat No (Assay ID)​
DMH0000256
Species​
Human (Homo sapiens)
Gene Symbol​
MET
Gene aliases​
HGFR;RCCP2;DFNB97
Ensembl Gene ID​
ENSG00000105976
Entrez Gene ID​
4233
Genomic Mutation ID (COSV by COSMIC)​
COSV59264428
Legacy Mutation ID (COSM by COSMIC)​
COSM735
Amino Acid Change​
p.?
Nucleotide Change​
c.3083-52_3083-51insCT
Wildtype Allele​
C
Mutant Allele​
CCT
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
TTTTTATCAAACTAATTCCATTATAAAAGCTCTTCCTGTTTCAGTCCCCATTAAATGAGGTTTTACTGTTGTTCTTTAATAATTTTCCTTCATCTTACAGATCAGTTTCCTAATTCATCTCAGAACGGTTCATGCCGACAAGTGCAG
Amplicon length​
127
Recommended Restriction Enzyme​
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX, ATTO550/ROX or Cy5/ATTO700
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC
Reaction size​
200rxns and 1000rxns

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 83.13 KBLanguage: English

Resources

Scientific Posters (2)
Standardized Preanalytical Stabilization of Human Saliva Prevents Genomic DNA Degradation and Allows for Detection of Rare Tumor Mutations Using dPCR
PDF (3MB)
Detection of rare events using the QIAcuity Digital PCR System
PDF (1MB)
Instrument User Manuals (1)
QIAcuity Application Guide
PDF (4MB)
Brochures & Guides (1)
Validated assays for the QIAcuity Digital PCR System Product Profile
PDF (2MB)
Quick-Start Protocols (1)
dPCR LNA Mutation Assays Quick-Start Protocol
PDF (72KB)
Safety Data Sheets (1)
Safety Data Sheets
Application Notes (1)
Liquid biopsy-based detection of PIK3CA mutations from cfDNA using an end-to-end digital PCR workflow
PDF (2MB)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Certificates of Analysis
Introducing the dPCR Mutation Assay MET 735 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay MET 735 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay MET 735 Human and elevate your work to new heights.
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