GeneGlobe ID: DMH0000273 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay EGFR 13558 Human

AA Mutation: p.A767_V769dup
Nucleotide Mutation: c.2309_2310delinsCCAGCGTGGAT
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay EGFR 13558 Human
GeneGlobe Cat No (Assay ID)​
DMH0000273
Species​
Human (Homo sapiens)
Gene Symbol​
EGFR
Gene aliases​
ERBB1;ERRP;ERBB
Ensembl Gene ID​
ENSG00000146648
Entrez Gene ID​
1956
Genomic Mutation ID (COSV by COSMIC)​
COSV51775806
Legacy Mutation ID (COSM by COSMIC)​
COSM13558
Amino Acid Change​
p.A767_V769dup
Nucleotide Change​
c.2309_2310delinsCCAGCGTGGAT
Wildtype Allele​
GAC
Mutant Allele​
GCCAGCGTGGAT
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
TGCCTCTCCCTCCCTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACC
Amplicon length​
71
Recommended Restriction Enzyme​
CviQI,AluI,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)
Introducing the dPCR Mutation Assay EGFR 13558 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay EGFR 13558 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay EGFR 13558 Human and elevate your work to new heights.