dPCR Mutation Assay IDH2 33731 Human

Product Specification

AA Mutation: p.R172G
Nucleotide Mutation: c.514A>G
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay IDH2 33731 Human

GeneGlobe Cat No (Assay ID)

DMH0000282

Species

Human (Homo sapiens)

Gene Symbol

IDH2

Gene aliases

IDH-2

Ensembl Gene ID

ENSG00000182054

Entrez Gene ID

3418

Genomic Mutation ID (COSV by COSMIC)

COSV57468989

Legacy Mutation ID (COSM by COSMIC)

COSM33731

Amino Acid Change

p.R172G

Nucleotide Change

c.514A>G

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

GATGGCTAGGCGAGGAGCTCCAGTCGGGGGGTGCCCAGGTCAGTGGATCCCCTCTCCACCCTGGCCTACCTGGTCGCCATGGGCGTGCCTGCCAATGGTGATGGGCTTGGTCCAGCCAGGGACTAGG

Amplicon length

107

Recommended Restriction Enzyme

CviQI,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

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dPCR Mutation Assay IDH2 33731 Human

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Product Specification

Resources

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