dPCR Mutation Assay EGFR 12382 Human | GeneGlobe

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Product Specification

AA Mutation: p.L747_A750delinsP
Nucleotide Mutation: c.2239_2248delinsC
dPCR wet-lab validated

Assay Name

dPCR Mutation Assay EGFR 12382 Human

GeneGlobe Cat No (Assay ID)

DMH0000304

Species

Human (Homo sapiens)

Gene Symbol

EGFR

Gene aliases

ERBB1;ERRP;ERBB

Ensembl Gene ID

ENSG00000146648

Entrez Gene ID

1956

Genomic Mutation ID (COSV by COSMIC)

COSV51765099

Legacy Mutation ID (COSM by COSMIC)

COSM12382

Amino Acid Change

p.L747_A750delinsP

Nucleotide Change

c.2239_2248delinsC

Wildtype Allele

ATTAAGAGAAG

Mutant Allele

AC

Mutation Strand

+

Mutant description

Substitution - Missense

Control Sequence (wt)

TTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGG

Amplicon length

142

Recommended Restriction Enzyme

CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX, ATTO550/ROX or Cy5/ATTO700

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

200rxns and 1000rxns

Resources

Download Safety Data Sheets for QIAGEN product components.

Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis

Introducing the dPCR Mutation Assay EGFR 12382 Human, a cutting-edge addition to our dPCR LNA Mutation Assays lineup, specifically designed for Mutation/Variant Detection applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR Mutation Assay EGFR 12382 Human facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Mutation/Variant Detection, or any other sophisticated analyses, this product from our dPCR LNA Mutation Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR Mutation Assay EGFR 12382 Human and elevate your work to new heights.

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