dPCR Mutation Assay NRAS 575 Human

Product Specification

Assay Name

GeneGlobe Cat No (Assay ID)

DMH0000345

Species

Human (Homo sapiens)

Gene Symbol

NRAS

Gene aliases

N-ras

Ensembl Gene ID

ENSG00000213281

Entrez Gene ID

4893

Genomic Mutation ID (COSV by COSMIC)

COSV54736793

Legacy Mutation ID (COSM by COSMIC)

COSM575

Amino Acid Change

p.G13A

Nucleotide Change

c.38G>C

Wildtype Allele

Mutant Allele

Mutation Strand

Mutant description

Substitution - Missense

Positive Control Sequence

TGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACCTGCTCCAACCACCACCAGTTTGTACTCAGTCATTTCACACCAGCAAGAACCTGTTGGAAACCAGTA

Amplicon length

Recommended Restriction Enzyme

HaeIII,EcoRI,XbaI,PvuII

Wet-lab validated

dPCR wet-lab validated

Probe Fluorophore

FAM/HEX or ATTO550/ROX

Quencher

Iowa Black

Primer Purification

Desalted

Probe Purification

HPLC

Resources

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.

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dPCR Mutation Assay NRAS 575 Human

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Product Specification

Resources

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