Assay Name
dPCR Mutation Assay TP53 220778 Human
GeneGlobe Cat No (Assay ID)
DMH0000475
Species
Human (Homo sapiens)
Gene Symbol
TP53
Gene aliases
p53;LFS1
Ensembl Gene ID
ENSG00000141510
Entrez Gene ID
7157
Genomic Mutation ID (COSV by COSMIC)
COSV52680378
Legacy Mutation ID (COSM by COSMIC)
COSM220778
Amino Acid Change
p.R158H
Nucleotide Change
c.473G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
AACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGCGGGGGTGTGGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACATCTTGTTGAGGGCAGGGG
Amplicon length
117
Recommended Restriction Enzyme
CviQI,EcoRI,XbaI
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC