Name: dPCR RNA-Plex Assay Human RASGRF2
Brand: QIAGEN

Specification

Resources

Protocol for quantification from RNA templates

September 2026

Protocol for quantification from cDNA templates

September 2026

Download Safety Data Sheets for QIAGEN product components.

This five-step guide helps you select suitable reference genes, so you can incorporate the appropriate normalization into your gene expression analysis.

This app note demonstrates how the fundamentally different approach of dPCR, compared to qPCR, reduces susceptibility to amplification-related bias resulting in high-level multiplexing experiments that are simpler, more cost-effective and produce more precise data.

This app note demonstrates that dPCR offers improved accuracy and resolution of small changes in gene expression, compared to qPCR. The system delivers superior reproducibility across different operators, especially when detecting low-abundance targets or subtle expression changes.

These guidelines provide a step-by-step workflow for establishing a higher-order multiplex dPCR assay using the dPCR RNA-Plex Assays and the QIAcuity OneStep High Multiplex PCR Kit.

You may also need

QIAcuity High Multiplex Probe PCR Kit (1mL)

Cat. No.: 250133

1mL 4x concentrated QIAcuity High Multiplex Probe PCR Master Mix, 2 x 1.9mL RNAse-free Water, QN Internal Control DNA

QIAcuity High Multiplex Probe PCR Kit (5mL)

Cat. No.: 250134

5 x 1mL 4x concentrated QIAcuity High Multiplex Probe PCR Master Mix, 8 x 1.9mL RNAse-free Water, QN Internal Control DNA

QIAcuity OneStep High Multiplex PCR Kit (1 mL)

Cat. No.: 250135

1mL 4x OneStep High Multiplex PCR Master Mix, 45µl 100x OneStep High Multiplex RT Mix, 1ml Enhancer GC, 2 x 1.9mL RNAse-free Water, 20µl QN Internal Control RNA; for 100 reactions in Nanoplate 26K and 333 reactions in Nanoplate 8.5K

QIAcuity OneStep High Multiplex PCR Kit (5 mL)

Cat. No.: 250136

5 x 1mL 4x OneStep High Multiplex PCR Master Mix, 5 x 45µl 100x OneStep High Multiplex RT Mix, 5x 1ml Enhancer GC, 8 x 1.9mL RNAse-free Water, 20µl QN Internal Control RNA; for 500 reactions in Nanoplate 26K and 1666 reactions in Nanoplate 8.5K

QIAcuity OneStep Advanced Probe Kit (5 ml)

Cat. No.: 250132

5 x 1 ml OneStep Advanced Probe Master Mix (4x), 5 x 45 µl OneStep RT Mix (100x), 5 x 1 ml Enhancer GC, 1 x 20 µl QN Internal Control RNA, 8 x 1.9 ml RNase-free water; for 500 reactions in Nanoplate 26K and 1666 reactions in Nanoplate 8.5K