GeneSolution for Entrez gene 5873 consists of:
SI02662744 (FlexiTube siRNA)
SI03050075 (FlexiTube siRNA)
SI02633603 (FlexiTube siRNA)
SI00065541 (FlexiTube siRNA)
Name
FlexiTube GeneSolution GS5873 for RAB27A
Official Name
RAB27A, member RAS oncogene family
Species
Human (Homo sapiens)
RefSeq ID
NM_004580, NM_183235, NM_183234, NM_183236
Species
Human (Homo sapiens)
Official Name
RAB27A, member RAS oncogene family
Detected Transcripts
NM_004580 (3474 bp), NM_183235 (3464 bp), NM_183234 (3455 bp), NM_183236 (3415 bp)
Potential Off - Target mRNAs, 3' UTR Seed Match
219333|NM_182488|AS
Current Target mRNAs
5873|NM_004580 // 5873|NM_183235 // 5873|NM_183234 // 5873|NM_183236
Species
Human (Homo sapiens)
Official Name
RAB27A, member RAS oncogene family
Detected Transcripts
NM_004580 (3474 bp), NM_183234 (3455 bp), NM_183236 (3415 bp), NM_183235 (3464 bp)
Potential Off - Target mRNAs, 3' UTR Seed Match
23426|NM_001178074|AS // 23426|NM_021150|AS // 79645|NM_001142857|AS // 58508|NM_170606|S
Current Target mRNAs
5873|NM_004580 // 5873|NM_183234 // 5873|NM_183236 // 5873|NM_183235
Experimental Details
75% Knockdown
Cell Line: HeLa S3
26
Concentration of transfected siRNA: 25 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin
Species
Human (Homo sapiens)
Official Name
RAB27A, member RAS oncogene family
Detected Transcripts
NM_183236 (3415 bp), NM_004580 (3474 bp), NM_183234 (3455 bp), NM_183235 (3464 bp)
Potential Off-Target mRNAs, High Homology
57471|NM_001009959|3645|AS // 57471|NM_020711|3713|AS
Potential Off - Target mRNAs, 3' UTR Seed Match
11010|NM_006851|AS // 55332|NM_018370|AS // 81031|NM_030777|AS // 116931|NM_053002|AS // 135114|NM_138571|AS // 643155|NM_001048249|AS // 6731|NM_006947|S // 8434|NM_021111|S // 8500|NM_003626|S // 9204|NM_007167|S // 23271|NM_203459|S // 80205|NM_025134|S // 163131|NM_001005851|S
Current Target mRNAs
5873|NM_183236 // 5873|NM_004580 // 5873|NM_183234 // 5873|NM_183235
Species
Human (Homo sapiens)
Official Name
RAB27A, member RAS oncogene family
Detected Transcripts
NM_004580 (3474 bp), NM_183235 (3464 bp), NM_183234 (3455 bp), NM_183236 (3415 bp)
Potential Off-Target mRNAs, High Homology
91137|NM_138773|1876|AS
Current Target mRNAs
5873|NM_004580 // 5873|NM_183235 // 5873|NM_183234 // 5873|NM_183236