qBiomarker™ Somatic Mutation PCR Array Human Skin Cancers
GeneGlobe ID: SMH-024AA | Cat. No.: 337021 | qBiomarker Somatic Mutation PCR Arrays
Product Specification
PCR plate and master mix
Target List
BRAF
Common point mutations in BRAF either increase kinase activity, such as L597V, L597Q and V600E, or inhibit kinase activity, such as G464V, G466V and G469A.
CDKN2A
The most important CDKN2A loss-of-function mutations occur in the consensus ankyrin domain, which lead to the inability to form stable complexes with its targets.
CTNNB1
The most frequently detected CTNNB1 mutations result in abnormal WNT signaling activity. The mutated codons are mainly several serines and threonines targeted for phosphorylation by GSK3B.
FGFR3
The most frequently identified FGFR mutations occur in their kinase domains and non-kinase extracellular domains such as the hinge regions and IgG-like domains. Some of the somatic mutations also correspond to congenital SNPs involved in genetic diseases.
GNAQ
The mutations queried by these assays all lie in the protein's GTP nucleotide binding domain.
HRAS
The mutation assays detect the most important HRAS variants, all found in codons 12, 13, and 61.
KIT
The most frequently identified KIT gain-of-function mutations include the D816V point mutation; point mutations in, and a deletion of, exon 11 (the juxtamembrane domain); an exon 9 insertion; as well as exon 13 point mutations.
KRAS
The mutation assays detect the most frequently occurring KRAS mutations in codons 12, 13, and 61. Mutations at these positions reduce the protein’s intrinsic GTPase activity and/or cause it to become unresponsive to RasGAP.
NRAS
The mutation assays detect the most important NRAS variants, all found in codons 12, 13, and 61.
PIK3CA
The most frequently detected PIK3CA gain-of-function mutations occur either in the kinase domain (T1025-G1049, such as H1047L and H1047R) that increase its activity or in the helical domain (P539-E545) which mimic activation by growth factors.
PTCH1
The detected PTCH1 mutations constitutively activate the Hedgehog signaling pathway and the GLI transcription factors, which increases gene expression involved in tumor cell growth, differentiation, and proliferation.
PTEN
The most commonly detected PTEN loss-of-function mutations either truncate the protein (such as K6fs*4, R130*, R130fs*4, R233*, P248fs*5, and V317fs*3) or cause phosphatase inactivation (such as those at R130 and R173).
RB1
The most recurrent loss-of-function mutations in the RB1 tumor suppressor gene either truncate the protein or disrupt interactions with its binding partners.
SMO
The detected SMO mutation constitutively activates the Hedgehog signaling pathway and the GLI transcription factors, which increases gene expression involved in tumor cell growth, differentiation, and proliferation.
STK11
The most commonly detected STK11 inactivation variants either are point mutations or truncate the protein.
TP53
The most frequently detected somatic TP53 mutations occur in the DNA-binding domain which disrupt DNA binding and/or protein structure.
Product Resources
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Resources
Download Files (1)
Data analysis file for qBiomarker™ Somatic Mutation PCR Array Human DNA QC Pathway- FFPE Samples
Catalog number- 337021
Pathway number- SMH-999
Catalog number- 337021
Pathway number- SMH-999
Kit Handbooks (1)
For real-time PCR-based, pathway- or disease-focused somatic mutation profiling
Protocols (8)
User Manuals (2)
For screening disease-focused mutation panels by PCR
For gene expression and genomic analysis
White Papers (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Introducing the qBiomarker™ Somatic Mutation PCR Array Human Skin Cancers, a cutting-edge addition to our qBiomarker Somatic Mutation PCR Arrays lineup, specifically designed for Mutation/Variant Detection applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the qBiomarker™ Somatic Mutation PCR Array Human Skin Cancers facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Mutation/Variant Detection, or any other sophisticated analyses, this product from our qBiomarker Somatic Mutation PCR Arrays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with qBiomarker™ Somatic Mutation PCR Array Human Skin Cancers and elevate your work to new heights.