QIAseq miRNA Library Kit

Gel-free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next-generation sequencing

Gel-free miRNA sequencing library prep speeds up library construction time
Elimination of adapter dimers and hY4 RNA resulting in the highest fidelity and most efficient data from low input serum, plasma and exosome samples
Integrated Unique Molecular Indices (UMIs) enable quantification of individual miRNA molecules
Includes access to the RNA-seq Analysis Portal for human, mouse and rat samples
Available with 768 unique dual indices for high-throughput sequencing on Illumina NovaSeq instruments

Library Kit

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.

CAT NO./ID 331502∕-

QIAseq miRNA Library Kit (12)

For 12 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers

INQUIRE

CAT NO./ID 331505∕-

QIAseq miRNA Library Kit (96)

For 96 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers

$9,570.00

miRNA Index Kit Version 1

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.

CAT NO./ID 331592∕-

QIAseq miRNA 12 Index IL (12)

Sequencing adapters, primers and indexes compatible with Illumina platforms; 12 indexes for 12 samples

INQUIRE

CAT NO./ID 331595∕-

QIAseq miRNA 48 Index IL (96)

Sequencing adapters, primers and indexes compatible with Illumina platforms; two 48 indexes for 96 samples

$1,973.00

CAT NO./ID 331565∕-

QIAseq miRNA 96 Index IL (96)

Sequencing adapters, primers and indices compatible with Illumina platforms; 96 indices for 96 samples (pre-plated in 96-well plate for ease of use)

$1,973.00

CAT NO./ID 331601∕-

QIAseq miRNA 12 Index Kit IL UDI (12)

The QIAseq miRNA 12 Index Kit UDI is for indexing 12 samples using QIAseq miRNA library Kits. The sample indices are supplied as a liquid, in 96-well hard plastic plates, along with the UDI 5' Adapter (96) and the UDI RT Initiator (96). NOTE: In order to ensure data consistency, we recommend that all samples in a data set have UDIs. This ensures that the protocol and sequencing will be the same for all samples. Compatible UDI indices are 331601, 331615, 331625, 331635, 331645, 331655, 331665, 331675, 331685, 331717, 331727 and 331738

$804.00

CAT NO./ID 331582∕-

QIAseq miRNA 12 Index TF (12)

Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; 12 indices for 12 samples

INQUIRE

CAT NO./ID 331585∕-

QIAseq miRNA 48 Index TF (96)

Sequencing adapters, primers and indices compatible with Thermo Fisher platforms; two 48 indices for 96 samples

$1,973.00

miRNA Index Kit UDI Version 2

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.

Resources

For use with QIAseq miRNA library kits (331502, 331505) and QIAseq miRNA Index Kits UDI (331601, 331615, 331625, 331635, 331645, 331655, 331665, 331675, 331685, 331905, 331915, 331895, 331925, 331935, 331945, 331955, 331965, 331977, 331987, 331998)

For use with QIAseq miRNA library kits (331502, 331505) and Singular Genomics UDI Primer kit

For use with QIAseq miRNA library kits (331502, 331505) and QIAseq miRNA Index TF Kits (331582, 331585)

For use with QIAseq miRNA Library Auto Kit (384) (331509) and QIAseq miRNA 96 Index IL Auto A (331567, 331569)

For use with QIAseq miRNA library kits (331502, 331505) and QIAseq miRNA Index IL Kits (331592, 331595, 331565)

Part 2: QIAseq miRNA NGS (QMN) Bead preparation, cDNA cleanup

Part 1: 3' Ligation, 5' ligation, reverse transcription

Poster for download